Spectrins are highly conserved molecules that form membrane-associated periodic scaffolds composed of tetramers of two α-subunits and two ß-subunits. This scaffold provides mechanical resistance to cells and tissues. We have recently shown using C. elegans that UNC-70/ß-Spectrin forms a membrane-associated periodic scaffold in the epidermis associated with neurons to maintain the integrity of the associated sensory axons. Our research aims to investigate whether spectrin exhibits similar non-cell autonomous functions in other cell types that interact with neurons, such as glia. In humans, neuron-glia interactions are essential for brain development and function. Glia dysfunction has been linked to various neurological conditions, including neurodegenerative diseases, autism, epilepsy, and stroke. C. elegans serves as an ideal model to study the role of glial spectrin and contains only 56 glial cells associated with sensory neurons, many of which share similarities with vertebrate astrocytes in terms of their morphology, function, and gene expression. Here, we used sophisticated genetic approaches to systematically remove UNC-70/ß-Spectrin from all glia, as well as specific classes of glia, to determine its role in the development and maintenance of glia-neuron sense organs in C. elegans. In parallel, we also characterised the localisation and organisation of the spectrin network, at nanoscale resolution, in glia using super-resolution microscopy. Our results shed light on the cell biology of glia and their roles in neuronal development and maintenance.